Minimally destructive sampling of type specimens of Pyropia (Bangiales, Rhodophyta) recovers complete plastid and mitochondrial genomes
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Authors
Hughey, Jeffery R.
Gabrielson, Paul W.
Rohmer, Laurence
Tortolani, Jacquie
Silva, Mayra
Miller, Kathy Ann
Young, Joel D.
Martell, Craig
Ruediger, Erik
Subjects
Advisors
Date of Issue
2014-06-04
Date
Publisher
Language
Abstract
Plant species, including algae and fungi, are based on type specimens to which the name of a taxon is
permanently attached. Applying a scientific name to any specimen therefore requires demonstrating
correspondence between the type and that specimen. Traditionally, identifications are based on
morpho-anatomical characters, but recently systematists are using DNA sequence data. These studies are
flawed if the DNA is isolated from misidentified modern specimens. We propose a genome-based solution.
Using 4x4 mm² of material from type specimens, we assembled 14 plastid and 15 mitochondrial genomes
attributed to the red algae Pyropia perforata, Py. fucicola, and Py. kanakaensis. The chloroplast genomes
were fairly conserved, but the mitochondrial genomes differed significantly among populations in content
and length. Complete genomes are attainable from 19th and early 20th century type specimens; this validates
the effort and cost of their curation as well as supports the practice of the type method.
Type
Article
Description
The article of record as published may be found at http://dx.doi.org/10.1038/srep05113
Series/Report No
Department
Computer Science (CS)
Organization
Naval Postgraduate School (U.S.)
Identifiers
NPS Report Number
Sponsors
P.W.G. and
Dr. Kelly Locke, Title V grant director at Hartnell College
Funder
Grant number- PO31C110168
Format
Citation
Hughey, J.R. et al. Minimally destructive sampling of type
specimens of Pyropia (Bangiales, Rhodophyta) recovers complete plastid and
mitochondrial genomes. Sci. Rep. 4, 5113; DOI:10.1038/srep05113 (2014).
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This publication is a work of the U.S. Government as defined in Title 17, United States Code, Section 101. Copyright protection is not available for this work in the United States.