Effect of Transforming Growth Factor Beta (TGF Beta) and Vitamin D3 Metabolites on Protein Kinase C Mediated Signal Transduction in Rat Costochondral Chondrocyte Cultures.
Mackey, Scott A.
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It is Imown that vitamin D3 metabolites and transforming growth factor % (TOF-J3) regulate proliferation and differentiation of chondrocytes isolated from the growth zone or resting zone of rat costochondral cartilage. It was hypothesized that both TGF-% and the vitamin D metabolites play a role in signal transduction in the rat costochondral chondrocyte model and that the regulation of protein kinase C by these metabolites would be cell maturation- dependent. Confluent, fourth passage cultures of growth zone and resting zone chondrocytes were treated with vitamin D3 metabolites for up to 24 hours, lysed, and the cell extracts assayed for protein kinase C (PKC) specific activity using a specific peptide substrate. The addition of i,25-(OH)2D3 to growth zone chondrocyte cultures resulted in a rapid, dose dependent stimulation of PKC specific activity.
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