NPS logo Naval Postgraduate School
Dudley Knox Library
        View Item 
        •   Calhoun Home
        • Theses and Dissertations
        • 1. Thesis and Dissertation Collection, all items
        • View Item
        •   Calhoun Home
        • Theses and Dissertations
        • 1. Thesis and Dissertation Collection, all items
        • View Item
        • How to search in Calhoun
        • My Accounts
        • Ask a Librarian
        JavaScript is disabled for your browser. Some features of this site may not work without it.

        Browse

        All of CalhounCollectionsThis Collection

        My Account

        LoginRegister

        Statistics

        Most Popular ItemsStatistics by CountryMost Popular Authors

        A: The progression of a catalytic immune response; B: molecular recognition of anions by silica bound sapphyrin

        Thumbnail
        View/Open
        Iconaprogressionofca00thom.pdf (7.115Mb)
        Download Record
        Download to EndNote/RefMan (RIS)
        Download to BibTex
        Author
        Thomas, Richard Edward
        Date
        1994-08
        Advisor
        Iverson, Brent L.
        Sessler, Jonathan L.
        Metadata
        Show full item record
        Abstract
        Part A: This thesis describes the first detailed study of how a catalytic immune response matures over a series of immunizations. Specifically, three male New Zealand White rabbits were immunized with a 4- (carboxy) benzyl phenyl phosphate hapten conjugated to keyhole limpet hemocyanin (KLH) . The three rabbits received an initial immunization with the immunogen, followed by three immunizations at three week intervals. Serum samples were obtained from each rabbit ten days following every immunization. Pure polyclonal IgG antibodies were obtained by a multiple step isolation and purification process. The catalytic activity of each sample was demonstrated by the rate enhancement and catalytic efficiency of the hydrolysis of the corresponding carbonate substrate. The results show a remarkable similarity in the immune response maturation seen in two of the three rabbits. The third rabbit was diseased and lagged slightly behind. Through the four immunizations, the immune response continued to optimize due to an apparent increase in the affinity of the polyclonal antibodies.This is quite significant, in that it provides the first experimental evidence that affinity maturation is important for antibody catalysis. We have thus begun to shed the first rays of light inside the black box that produces catalytic antibodies. Part B: A novel sapphyrin-functionalized silica gel has been used to study the anionic binding properties of the expanded porphyrin sapphyrin. The preparation of the first generation of this type of support is discussed. The silica-bound sapphyrin displayed longer retention times under neutral, isochratic HPLC conditions, for molecules with sterically accessible oxyanions. In particular, the sapphyrin-functionalized silica gel showed a remarkable preference for arsonates and phosphates. Furthermore, mixtures of nucleotides and oligonucleotides were cleanly separated according to the number of phosphate units they possessed. Thus, not only does sapphyrin-bound silica gel provide an exquisite way to investigate anion binding specificities of sapphyrin, it also provides a viable method to selectively separate and analyze anion types. These types of modified silica gels may be useful for solving present day environmental problems, as well as separating and analyzing phosphorylated biologically relevant molecules.
        URI
        http://hdl.handle.net/10945/25701
        Collections
        • 1. Thesis and Dissertation Collection, all items

        Related items

        Showing items related by title, author, creator and subject.

        • Thumbnail

          A preliminary investigation of catalytic combustion under flow conditions 

          Lowell, Arthur C. (Troy, New York; Rensselaer Polytechnic Institute, 1949);
          In presenting the Conclusions and Summary, it was sought to satisfy the initial object of this paper in presenting indications as to the uses which can be made of catalytic combustion in heat production. The tests provided ...
        • Thumbnail

          Correlation immunity, avalanche features, and other cryptographic properties of generalized Boolean functions 

          Martinsen, Thor (Monterey, California: Naval Postgraduate School, 2017-09);
          This dissertation investigates correlation immunity, avalanche features, and the bent cryptographic properties for generalized Boolean functions defined on Vn with values in Zԛ. We extend the concept of correlation immunity ...
        • Thumbnail

          An Experimental Investigation into NOx Control of a Gas Turbine Combustor and Augmentor Tube Incorporating a Catalytic Reduction System 

          Behrens, Christopher Karl (Monterey, California: Naval Postgraduate School., 1990-03);
          An initial experimental investigation was conducted to examine the feasibility of NO x emission control using catalytic reduction techniques in the jet engine test cell environment. A modified T-63 gas turbine combustor ...
        Feedback

        411 Dyer Rd. Bldg. 339
        Monterey, CA 93943

         

        circdesk@nps.edu
        (831) 656-2947
        DSN 756-2947

        Start Your Research

        • Research Guides
        • How to Cite
        • Search Basics
        • Ask a Librarian
        • Library Liaisons
        • Graduate Writing Center
        • Thesis Processing Office
        • Statistics, Maps & More
        • Copyright at NPS

        Find & Download

        • Databases List
        • Articles, Books & More
        • NPS Theses
        • NPS Faculty Publications: Calhoun
        • Journal Titles
        • Course Reserves

        Use the Library

        • My Accounts
        • Request Article or Book
        • Borrow, Renew, Return
        • Remote Access
        • Workshops & Tours
        • For Faculty & Researchers
        • For International Students
        • For Alumni
        • Print, Copy, Scan, Fax
        • Rooms & Study Spaces
        • Floor Map
        • Computers & Software
        • Adapters, Lockers & More

        Collections

        • NPS Archive: Calhoun
        • Restricted Resources
        • Special Collections & Archives
        • Federal Depository
        • Homeland Security Digital Library

        About

        • Hours
        • Library Staff
        • About Us
        • Visit Us

        NPS-Licensed Resources - Terms & Conditions

        Copyright Notice

         
         

          Federal Depository Library  

        NPS Home Privacy Policy Copyright Accessibility Contact Webmaster