Metabolic effect of an exogenous gene on transgenic beauveria bassiana using liquid chromatography-mass spectrometry-based metabolomics
Author
Zhou, Hong
Luo, Feifei
Lu, Ruili
Hu, Fenglin
Bao, Guanhu
Huang, Bo
Li, Zengzhi
Date
2013Metadata
Show full item recordAbstract
Genetic modification of Beauveria bassiana with the scorpion neurotoxin aaIT gene can distinctly increase its
insecticidal activity, whereas the effect of this exogenous gene on the metabolism of B. bassiana is unknown until now. Thus,
we investigate the global metabolic profiling of mycelia and conidia of transgenic and wild-type B. bassiana by liquid
chromatography−mass spectrometry (LC−MS). Principal component analysis (PCA) and orthogonal projection to latent
structure discriminant analysis (OPLS-DA) reveal clear discrimination of wild-type mycelia and conidia from transgenic mycelia
and conidia. The decrease of glycerophospholipids, carnitine, and fatty acids and the increase of oxylipins, glyoxylate, pyruvic
acid, acetylcarnitine, fumarate, ergothioneine, and trehalose in transgenic mycelia indicate the enhanced oxidative reactions.
In contrast, most metabolites related to oxidative stress are not altered significantly in conidia, which implies that there will be no
significant oxidative stress reaction when the aaIT gene is quiescent in cells.
Description
Journal of Agricultural and Food Chemistry, 61(28), pages 7008-7017, 2013
The article of record as published may be found at http://dx.doi.org/10.1021/jf401703b