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dc.contributor.authorZhou, Hong
dc.contributor.authorLuo, Feifei
dc.contributor.authorLu, Ruili
dc.contributor.authorHu, Fenglin
dc.contributor.authorBao, Guanhu
dc.contributor.authorHuang, Bo
dc.contributor.authorLi, Zengzhi
dc.date2013
dc.date.accessioned2014-04-23T20:35:24Z
dc.date.available2014-04-23T20:35:24Z
dc.date.issued2013
dc.identifier.citationMetabolic effect of an exogenous gene on transgenic beauveria bassiana using liquid chromatography-mass spectrometry-based metabolomics (with F. Luo et al.), Journal of Agricultural and Food Chemistry, 61(28), pages 7008-7017, 2013
dc.identifier.urihttp://hdl.handle.net/10945/40942
dc.descriptionJournal of Agricultural and Food Chemistry, 61(28), pages 7008-7017, 2013en_US
dc.descriptionThe article of record as published may be found at http://dx.doi.org/10.1021/jf401703ben_US
dc.description.abstractGenetic modification of Beauveria bassiana with the scorpion neurotoxin aaIT gene can distinctly increase its insecticidal activity, whereas the effect of this exogenous gene on the metabolism of B. bassiana is unknown until now. Thus, we investigate the global metabolic profiling of mycelia and conidia of transgenic and wild-type B. bassiana by liquid chromatography−mass spectrometry (LC−MS). Principal component analysis (PCA) and orthogonal projection to latent structure discriminant analysis (OPLS-DA) reveal clear discrimination of wild-type mycelia and conidia from transgenic mycelia and conidia. The decrease of glycerophospholipids, carnitine, and fatty acids and the increase of oxylipins, glyoxylate, pyruvic acid, acetylcarnitine, fumarate, ergothioneine, and trehalose in transgenic mycelia indicate the enhanced oxidative reactions. In contrast, most metabolites related to oxidative stress are not altered significantly in conidia, which implies that there will be no significant oxidative stress reaction when the aaIT gene is quiescent in cells.en_US
dc.rightsThis publication is a work of the U.S. Government as defined in Title 17, United States Code, Section 101. Copyright protection is not available for this work in the United States.en_US
dc.titleMetabolic effect of an exogenous gene on transgenic beauveria bassiana using liquid chromatography-mass spectrometry-based metabolomicsen_US
dc.contributor.departmentDepartment of Applied Mathematics
dc.subject.authorB. bassianaen_US
dc.subject.authortransgenic strainsen_US
dc.subject.authormetabolomicsen_US
dc.subject.authorLC−MSen_US
dc.subject.authorglycerophospholipiden_US
dc.subject.authoroxidative stressen_US
dc.subject.authormetabolic pathwayen_US
dc.subject.authorantioxidanten_US


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