Minimally destructive sampling of type specimens of Pyropia (Bangiales, Rhodophyta) recovers complete plastid and mitochondrial genomes
Hughey, Jeffery R.
Gabrielson, Paul W.
Miller, Kathy Ann
Young, Joel D.
MetadataShow full item record
Plant species, including algae and fungi, are based on type specimens to which the name of a taxon is permanently attached. Applying a scientific name to any specimen therefore requires demonstrating correspondence between the type and that specimen. Traditionally, identifications are based on morpho-anatomical characters, but recently systematists are using DNA sequence data. These studies are flawed if the DNA is isolated from misidentified modern specimens. We propose a genome-based solution. Using 4x4 mm² of material from type specimens, we assembled 14 plastid and 15 mitochondrial genomes attributed to the red algae Pyropia perforata, Py. fucicola, and Py. kanakaensis. The chloroplast genomes were fairly conserved, but the mitochondrial genomes differed significantly among populations in content and length. Complete genomes are attainable from 19th and early 20th century type specimens; this validates the effort and cost of their curation as well as supports the practice of the type method.
The article of record as published may be found at http://dx.doi.org/10.1038/srep05113
Showing items related by title, author, creator and subject.
Stress distribution in two circular cylinders intersecting at right angles under the influence of internal pressure Harmon, Leonard Edward (Pasadena, California; California Institute of Technology, 1949);This investigation is an experimental study of the stress distribution in two circular cylinders intersecting at right angles and acted on by internal pressure. Two specimens of the thick-wall category were tested to ...
Dew, Dwight D. (U.S. Naval Postgraduate School, 1986);This paper presents the studies on measurement techniques developed for the determination of strain-dependent damping characteristics of materials in an air environment. The material is a high damping manganese-copper ...
Electrical resistivity and Hall effect of cuprous sulfide and bismuth telluride in the temperature range from 290K to 670K. Erickson, Philip W. (Monterey, California: U.S. Naval Postgraduate School, 1961);An instrumentation and experimental technique for the measurements of electrical resistivity and the Hall effect at elevated temperatures is described. These two temperature-dependent properties are uniquely determined ...