Minimally destructive sampling of type specimens of Pyropia (Bangiales, Rhodophyta) recovers complete plastid and mitochondrial genomes

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Author
Hughey, Jeffery R.
Gabrielson, Paul W.
Rohmer, Laurence
Tortolani, Jacquie
Silva, Mayra
Miller, Kathy Ann
Young, Joel D.
Martell, Craig
Ruediger, Erik
Date
2014-06-04Metadata
Show full item recordAbstract
Plant species, including algae and fungi, are based on type specimens to which the name of a taxon is
permanently attached. Applying a scientific name to any specimen therefore requires demonstrating
correspondence between the type and that specimen. Traditionally, identifications are based on
morpho-anatomical characters, but recently systematists are using DNA sequence data. These studies are
flawed if the DNA is isolated from misidentified modern specimens. We propose a genome-based solution.
Using 4x4 mm² of material from type specimens, we assembled 14 plastid and 15 mitochondrial genomes
attributed to the red algae Pyropia perforata, Py. fucicola, and Py. kanakaensis. The chloroplast genomes
were fairly conserved, but the mitochondrial genomes differed significantly among populations in content
and length. Complete genomes are attainable from 19th and early 20th century type specimens; this validates
the effort and cost of their curation as well as supports the practice of the type method.
Description
The article of record as published may be found at http://dx.doi.org/10.1038/srep05113
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This publication is a work of the U.S. Government as defined in Title 17, United States Code, Section 101. Copyright protection is not available for this work in the United States.Collections
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